New CRISPR-EZ Method Makes Genome Editing Much Easier in Mice
May 31, 2016
(News-Medical) – To do that, they need to knock out or modify specific genes in lab animals – in particular, mice – and see what goes awry. Today’s gold standard for creating these “knockouts” or “knockins” is to edit the genes inside mouse embryonic stem cells, use these cells to create mosaic mice, and then crossbreed the mice to get a pure genetic strain. Because of CRISPR-Cas9’s ability to precisely alter or replace genes, the editing is increasingly being done directly in the fertilized egg, or early embryo. The new method, called CRISPR-EZ (CRISPR RNP electroporation of zygotes), makes genome editing in mouse embryos even easier.